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The Inactivation by Curcumin-Mediated Photosensitization of Botrytis cinerea Spores Isolated from Strawberry Fruits

Huang, L., Yong, K. W. L., Fernando, W. C., Carpinelli de Jesus, M., De Voss, J. J., Sultanbawa, Y. and Fletcher, M. T. (2021) The Inactivation by Curcumin-Mediated Photosensitization of Botrytis cinerea Spores Isolated from Strawberry Fruits. Toxins, 13 (3). ISSN 2072-6651

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Article Link(s): https://doi.org/10.3390/toxins13030196

Abstract

Photosensitization is a novel environmentally friendly technology with promising applications in the food industry to extend food shelf life. In this study, the natural food dye curcumin, when combined with visible light (430 nm), was shown to be an effective photosensitizer against the common phytopathogenic fungi Botrytis cinerea (the cause of grey mould). Production of the associated phytotoxic metabolites botrydial and dihydrobotrydial was measured by our newly developed and validated HRAM UPLC-MS/MS method, and was also shown to be reduced by this treatment. With a light dose of 120 J/cm2, the reduction in spore viability was directly proportional to curcumin concentrations, and the overall concentration of both botrydial and dihydrobotrydial also decreased with increasing curcumin concentration above 200 µM. With curcumin concentrations above 600 µM, the percentage reduction in fungal spores was close to 100%. When the dye concentration was increased to 800 µM, the spores were completely inactive and neither botrydial nor dihydrobotrydial could be detected. These results suggest that curcumin-mediated photosensitization is a potentially effective method to control B. cinerea spoilage, and also to reduce the formation of these phytotoxic botryane secondary metabolites.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:photodynamic therapy botryane secondary metabolites dihydrobotrydial fungal spoilage
Subjects:Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Plant culture > Harvesting, curing, storage
Plant culture > Food crops
Plant culture > Fruit and fruit culture > Berries and small fruits
Deposited On:16 Mar 2021 04:27
Last Modified:16 Mar 2021 04:27

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