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Cucumber mosaic virus infection transiently breaks dsRNA-induced transgenic immunity to Potato virus Y in tobacco

Mitter, N. and Sulistyowati, E. and Dietzgen, R.G. (2003) Cucumber mosaic virus infection transiently breaks dsRNA-induced transgenic immunity to Potato virus Y in tobacco. Molecular Plant-Microbe Interactions, 16 (10). pp. 936-944.

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Abstract

Post-transcriptional gene silencing (PTGS), an intrinsic plant defense mechanism, can be efficiently triggered by double stranded (ds)RNA-producing transgenes and can provide high level virus resistance by specific targeting of cognate viral RNA. The discovery of virus-encoded suppressors of PTGS led to concerns about the stability of such resistance.

Here, we show that Cucumber mosaic virus (CMV) is able to suppress dsRNA-induced PTGS and the associated Potato virus Y (PVY) immunity in tobacco. CMV suppression supported only a transient PVY accumulation and did not prevent recovery of the transgenic plants from PVY infection. CMV inoculation resulted in strongly increased transgene mRNA levels due to suppression of PTGS, but accumulation of PVY-specific small interfering (si)RNA was unaffected.

However, PVY accumulation in previously immune plants resulted in increased PVY siRNA levels and transgene mRNA was no longer detected, despite the presence of CMV. Transgene mRNA returned to high levels once PVY was no longer detected in CMVinfected plants. Recovered and chronically CMV-infected tissues were immune to further PVY infection.

Item Type:Article
Additional Information:© American Phytopathological Society is very pleased to be able to offer open access to APS journals after 24 months. Our preference is that you post the abstracts of articles published by DPI&F staff on your site with a direct link to the full article on APSnet.
Keywords:PTGS suppression; transgenic virus resistance.
Subjects:Plant pests and diseases > Individual or types of plants or trees
Science > Microbiology > Virology
Science > Biology > Genetics
Plant culture > Field crops > Other field crops
Deposited On:16 Jul 2004
Last Modified:04 Apr 2011 03:06

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