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Pilot-scale resin adsorption as a means to recover and fractionate apple polyphenols

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Kammerer, D.R., Carle, R., Stanley, R.A. and Saleh, Z.S. (2010) Pilot-scale resin adsorption as a means to recover and fractionate apple polyphenols. Journal of Agricultural and Food Chemistry, 58 (11). pp. 6787-6796. ISSN 00218561 (ISSN)

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Article Link: https://doi.org/10.1021/jf1000869


The purification and fractionation of phenolic compounds from crude plant extracts using a food-grade acrylic adsorbent were studied at pilot-plant scale. A diluted apple juice concentrate served as a model phenolic solution for column adsorption and desorption trials. Phenolic concentrations were evaluated photometrically using the Folin-Ciocalteu assay and by HPLC-DAD. Recovery rates were significantly affected by increasing phenolic concentrations of the feed solutions applied to the column. In contrast, the flow rate during column loading hardly influenced adsorption efficiency, whereas the temperature and pH value were shown to be crucial parameters determining both total phenolic recovery rates and the adsorption behavior of individual polyphenols. As expected, the eluent composition had the greatest impact on the desorption characteristics of both total and individual phenolic compounds. HPLC analyses revealed significantly different elution profiles of individual polyphenols depending on lipophilicity. This technique allows fractionation of crude plant phenolic extracts, thus providing the opportunity to design the functional properties of the resulting phenolic fractions selectively, and the present study delivers valuable information with regard to the adjustment of individual process parameters. © 2010 American Chemical Society.

Item Type:Article
Business groups:Crop and Food Science
Keywords:Adsorbent resin Adsorption Apple polyphenols Desorption Fractionation Recovery flavonoid phenol derivative plant extract polyphenols resin apple article chemistry evaluation high performance liquid chromatography instrumentation isolation and purification methodology Chromatography, High Pressure Liquid Flavonoids Malus Phenols Plant Extracts Resins, Synthetic Malus x domestica
Subjects:Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Plant culture > Fruit and fruit culture
Live Archive:16 Feb 2023 06:51
Last Modified:16 Feb 2023 06:51

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