Functional Soil Biological Measurements to Support Healthy Soils

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Lapis-Gaza, H. R. and Pattison, A. B. (2021) Functional Soil Biological Measurements to Support Healthy Soils. In: The Plant Microbiome: Methods and Protocols. Methods in Molecular Biology book series (MIMB, ) (V.2232). Springer US, New York, NY. ISBN 978-1-0716-1040-4

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Article Link: https://doi.org/10.1007/978-1-0716-1040-4_20

Abstract

Soil microorganisms contribute significantly to terrestrial ecosystem functioning through their activities. Various methods exist to characterize soil microbial activity and functional diversity including those that focus on potential enzyme activities and the respiratory responses of microbes to different substrates. Here, we describe: (1) the fluorescein diacetate hydrolysis assay for total potential microbial enzyme activity; (2) measurement of beta-glucosidase activity using ρ-nitrophenyl (pNP); (3) multienzyme assay using 4-methylumbelliferone (MUB); and (4) MicroResp assays to measure the respiratory responses of microbes to different substrates and generate a community level physiological profile (CLPP).

Item Type:	Book Section
Business groups:	Horticulture and Forestry Science
Keywords:	Total microbial activity Soil enzyme activity 4-Methylumbelliferone Fluorescein diacetate ρ-Nitrophenyl Enzyme assay Microbial functional diversity Beta-glucosidase
Subjects:	Science > Biology > Molecular Biology Agriculture > Agriculture (General) > Soils. Soil science Agriculture > Agriculture (General) > Soils. Soil science > Soil and crops. Soil-plant relationships. Soil productivity Plant culture Plant pests and diseases > Plant pathology
Live Archive:	12 Nov 2020 00:32
Last Modified:	09 Dec 2021 02:09

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