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Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia

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Guo, S., Wakeham, D., Brouwers, H. J. M., Cobbold, R. N., Abraham, S., Mollinger, J. L., Johnson, J. R., Chapman, T. A., Gordon, D. M., Barrs, V. R. and Trott, D. J. (2015) Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia. Microbes and Infection, 17 (4). pp. 266-274.

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Article Link: http://dx.doi.org/10.1016/j.micinf.2014.12.016

Publisher URL: http://www.sciencedirect.com/science/article/pii/S1286457914003384

Abstract

Phylogenetic group D extraintestinal pathogenic Escherichia coli (ExPEC), including O15:K52:H1 and clonal group A, have spread globally and become fluoroquinolone-resistant. Here we investigated the role of canine feces as a reservoir of these (and other) human-associated ExPEC and their potential as canine pathogens. We characterized and compared fluoroquinolone-resistant E. coli isolates originally identified as phylogenetic group D from either the feces of hospitalized dogs (n = 67; 14 dogs) or extraintestinal infections (n = 53; 33 dogs). Isolates underwent phylogenetic grouping, random amplified polymorphic DNA (RAPD) analysis, virulence genotyping, resistance genotyping, human-associated ExPEC O-typing, and multi-locus sequence typing. Five of seven human-associated sequence types (STs) exhibited ExPEC-associated O-types, and appeared in separate RAPD clusters. The largest subgroup (16 fecal, 26 clinical isolates) were ST354 (phylogroup F) isolates. ST420 (phylogroup B2); O1-ST38, O15:K52:H1-ST393, and O15:K1-ST130 (phylogroup D); and O7-ST457, and O1-ST648 (phylogroup F) were also identified. Three ST-specific RAPD sub-clusters (ST354, ST393, and ST457) contained closely related isolates from both fecal or clinical sources. Genes encoding CTX-M and AmpC β-lactamases were identified in isolates from five STs. Major human-associated fluoroquinolone-resistant ± extended-spectrum cephalosporin-resistant ExPEC of public health importance may be carried in dog feces and cause extraintestinal infections in some dogs.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Extraintestinal pathogenic Escherichia coli Fluoroquinolone-resistant Extended-spectrum cephalosporin-resistant Phylogenetic group D
Subjects:Science > Microbiology
Science > Microbiology > Microorganisms in the animal body
Veterinary medicine > Veterinary microbiology
Live Archive:13 Jul 2015 03:36
Last Modified:03 Sep 2021 16:50

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