Studies on serological differentiation of heated animal proteins

Abstract

The technique of preparing antisera heat-alkali-treated serum-antigens is described together with the elimination of cross-reacting "heat"-antibodies from such antisera. The prepared antisera showed a rather low potency against normal sera but possessed a high sensitivity against serum-antigens heated from 70 degrees to 100 degrees C. The absorbed (specific) antisera, however, were less satisfactory for differentiation of NaOH-extracted "insoluble" (boiled and powdered) serum-proteins than the unabsorbed (non-specific) antisera, and both failed completely to give precipitin reactions with cooked meat-proteins.