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Stable isotope dilution assay and HS-SPME-GCMS quantification of key aroma volatiles of Australian pineapple (Ananas comosus) cultivars

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George, J., Nguyen, T. T. L., Sanewski, G., Hardner, C. and Smyth, H. E. (2024) Stable isotope dilution assay and HS-SPME-GCMS quantification of key aroma volatiles of Australian pineapple (Ananas comosus) cultivars. Food Chemistry, 455 . p. 139956. ISSN 0308-8146

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Article Link: https://doi.org/10.1016/j.foodchem.2024.139956

Publisher URL: https://www.sciencedirect.com/science/article/pii/S0308814624016066


Pineapple aroma is one of the most important sensory quality traits that influences consumer purchasing patterns. Reported in this paper is a high throughput method to quantify in a single analysis the key volatile organic compounds that contribute to the aroma of pineapple cultivars grown in Australia. The method constituted stable isotope dilution analysis in conjunction with headspace solid-phase microextraction coupled with gas-chromatography mass spectrometry. Deuterium labelled analogues of the target analytes purchased commercially were used as internal standards. Twenty-six volatile organic compounds were targeted for quantification and the resulting calibration functions of the matrix -matched validated method had determination coefficients (R2) ranging from 0.9772 to 0.9999. The method was applied to identify the key aroma volatile compounds produced by popular pineapple cultivars such as ‘Aus Carnival’, ‘Aus Festival’, ‘Aus Jubilee’, ‘Aus Smooth (Smooth Cayenne)’ and ‘Aussie Gold (73-50)’, grown in Queensland, Australia. Pineapple cultivars varied in its content and composition of free volatile components, which were predominantly comprised of esters, followed by terpenes, alcohols, aldehydes, and ketones.

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Crop and Food Science, Horticulture and Forestry Science
Keywords:Pineapple Flavour Volatile organic compounds Aroma Gas chromatography–mass spectrometry (GC–MS) Head space - solid-phase microextraction (HS-SPME) Stable-isotope dilution analysis (SIDA)
Subjects:Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Plant culture > Food crops
Plant culture > Fruit and fruit culture
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Agriculture > By region or country > Australia > Queensland
Live Archive:06 Jun 2024 00:32
Last Modified:06 Jun 2024 00:32

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