A method for high-throughput image-based antifungal screening

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Sabburg, R., Gregson, A., Urquhart, A. S., Aitken, E. A. B., Smith, L., Thatcher, L. F. and Gardiner, D. M. (2021) A method for high-throughput image-based antifungal screening. Journal of Microbiological Methods, 190 . p. 106342. ISSN 0167-7012

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Article Link: https://doi.org/10.1016/j.mimet.2021.106342

Publisher URL: https://www.sciencedirect.com/science/article/pii/S0167701221002104

Abstract

Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.

Item Type:	Article
Business groups:	Crop and Food Science
Keywords:	Antifungal Verticillium Aspergillus Fusarium Rapid Susceptibility
Subjects:	Science > Microbiology Plant culture Plant pests and diseases
Live Archive:	21 Oct 2021 06:20
Last Modified:	21 Oct 2021 06:20

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