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Sampling Microbiomes Associated with Different Plant Compartments

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Birt, H. W. G., Pattison, A. B. and Dennis, P. G. (2021) Sampling Microbiomes Associated with Different Plant Compartments. In: The Plant Microbiome: Methods and Protocols. Springer US, New York, NY. ISBN 978-1-0716-1040-4

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Article Link: https://doi.org/10.1007/978-1-0716-1040-4_2


The microbiome is known to influence plant fitness and differs significantly between plant compartments. To characterize the communities associated with different plant compartments, it is necessary to separate plant tissues in a manner that is suitable for microbiome analysis. Here, we describe a standardized protocol for sampling the microbiomes associated with bulk soil, the apical and basal ectorhizosphere, the apical and ectorhizosphere, the rhizome, pseudostem, and leaves of Musa spp. The approach can easily be modified for work with other plants.

Item Type:Book Section
Business groups:Horticulture and Forestry Science
Keywords:Sampling Microbiome Plant Sequencing Bacteria Fungi Rhizosphere
Subjects:Science > Biology > Molecular Biology
Science > Botany > Plant physiology
Science > Botany > Genetics
Plant culture
Plant pests and diseases > Plant pathology
Live Archive:12 Nov 2020 00:26
Last Modified:03 Sep 2021 16:46

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