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Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting

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Corney, B. G., Colley, J., Djordjevic, S. P., Whittington, R. and Graham, G. C. (1993) Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting. Journal of clinical microbiology, 31 (11). pp. 2927-2932. ISSN 0095-11371098-660X

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Article Link(s): https://doi.org/10.1128/JCM.31.11.2927-2932.1993

Article URL: https://pubmed.ncbi.nlm.nih.gov/8263177
Article URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC266156/

Abstract

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Australian cattle. Each serovar and genotype had a unique RAPD profile. Of 26 field isolates of Leptospira, 23 were identified as hardjo genotype bovis subtype A, 2 were identified as zanoni, and 1 was identified as pomona by RAPD fingerprinting, and their types were confirmed by cross-absorption agglutination and restriction enzyme analysis.

Item Type:Article
Additional Information:Open access PDF attached
Keywords:Animals Cattle/*microbiology *DNA Fingerprinting DNA, Bacterial/*analysis Gene Amplification Leptospira/genetics/*isolation & purification Polymerase Chain Reaction
Subjects:Science > Biology > Genetics
Science > Microbiology
Animal culture > Cattle
Veterinary medicine > Veterinary microbiology
Deposited On:29 Sep 2020 04:56
Last Modified:29 Sep 2020 04:56

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