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Plasmid-encoded Tet B tetracycline resistance in Haemophilus parasuis

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Lancashire, J. F., Terry, T. D., Blackall, P. J. and Jennings, M. P. (2005) Plasmid-encoded Tet B tetracycline resistance in Haemophilus parasuis. Antimicrobial agents and chemotherapy, 49 (5). pp. 1927-1931. ISSN 0066-48041098-6596

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Article Link(s): https://doi.org/10.1128/AAC.49.5.1927-1931.2005

Article URL: https://pubmed.ncbi.nlm.nih.gov/15855515
Article URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087615/

Abstract

The complete sequence of two plasmids, pHS-Tet (5.1 kb) and pHS-Rec (9.5 kb), isolated from Haemophilus parasuis strain HS1543 has been obtained. Plasmid pHS-Tet contains four open reading frames including a tet(B) tetracycline resistance gene which unusually did not have an associated tetR repressor gene. From a total of 45 H. parasuis isolates surveyed (15 international reference strains, 15 field isolates selected for their genetic diversity, and 15 recent Australian field isolates), 2 tetracycline-resistant field isolates (HS226 and HS1859) were identified. Analysis of three additional isolates from the same disease outbreak as strain HS1859 revealed a further tetracycline-resistant H. parasuis strain (HS1857, serovar 8) and a tetracycline-resistant Actinobacillus pleuropneumoniae strain (HS1861). An approximately 10.6-kb plasmid was identified in field isolate HS226 and outbreak strains HS1857, HS1859, and HS1861. Southern hybridization analysis of these plasmids showed that the Tet B determinant was present, and restriction digest comparisons suggest that these plasmids are related. This is believed to be the first report of native H. parasuis plasmids and Tet B-mediated tetracycline resistance in this microorganism.

Item Type:Article
Additional Information:Open access PDF attached
Keywords:Actinobacillus pleuropneumoniae/drug effects/genetics Bacterial Proteins/*genetics Blotting, Southern Chromatography, Agarose DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics Electroporation Haemophilus/*drug effects/*genetics Microbial Sensitivity Tests Molecular Sequence Data Plasmids/*genetics Tetracycline Resistance/*genetics
Subjects:Science > Microbiology > Microorganisms in the animal body
Science > Microbiology > Bacteria
Veterinary medicine > Veterinary microbiology
Veterinary medicine > Veterinary bacteriology
Veterinary medicine > Diseases of special classes of animals
Veterinary medicine > Diseases of special classes of animals > Swine
Deposited On:29 Sep 2020 04:04
Last Modified:29 Sep 2020 04:04

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