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Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay

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Boyle, D. G., Boyle, D. B., Olsen, V., Morgan, J. A. T. and Hyatt, A. D. (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms, 60 (2). pp. 141-148.

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Article Link(s): https://doi.org/10.3354/dao060141

Publisher URL: http://www.int-res.com/abstracts/dao/v60/n2/p141-148/

Abstract

Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide, associated with declines in amphibian populations. Diagnosis of chytridiomycosis to date has largely relied upon histological and immunohistochemical examination of toe clips. This technique is invasive and insensitive particularly at early stages of infection when treatment may be possible. We have developed a real-time PCR Taqman assay that can accurately detect and quantify one zoospore in a diagnostic sample. This assay will assist the early detection of B. dendrobatidis in both captive and wild populations, with a high degree of sensitivity and specificity, thus facilitating treatment and protection of endangered populations, monitoring of pristine environments and preventing further global spread via amphibian trade.

Item Type:Article
Subjects:Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Veterinary medicine > Veterinary bacteriology
Veterinary medicine > Veterinary pathology
Deposited On:18 Aug 2020 23:56
Last Modified:18 Aug 2020 23:56

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