Login | Create Account (DAF staff only)

Gene expression in response to glyphosate treatment in fleabane (Conyza bonariensis) – glyphosate death response and candidate resistance genes

Hereward, J. P., Werth, J. A., Thornby, D. F., Keenan, M., Chauhan, B. S. and Walter, G. H. (2018) Gene expression in response to glyphosate treatment in fleabane (Conyza bonariensis) – glyphosate death response and candidate resistance genes. Pest Management Science, 74 (10). pp. 2346-2355. ISSN 1526498X (ISSN)

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://doi.org/http://doi.org/10.1002/ps.4804

Abstract

BACKGROUND: This study takes a whole-transcriptome approach to assess gene expression changes in response to glyphosate treatment in glyphosate-resistant fleabane. We assessed gene expression changes in both susceptible and resistant lines so that the glyphosate death response could be quantified, and constitutively expressed candidate resistance genes identified. There are three copies of the glyphosate target site (5-enolpyruvylshikimate-3-phosphate; EPSPS) gene in Conyza and because Conyza bonariensis is allohexaploid, there is a baseline nine copies of the gene in any individual. RESULTS: Many genes were differentially expressed in response to glyphosate treatment. Known resistance mutations are present in EPSPS2 but they are present in a glyphosate-susceptible line as well as resistant lines and therefore not sufficient to confer resistance. EPSPS1 is expressed four times more than EPSPS2, further reducing the overall contribution of these mutations. CONCLUSION: We demonstrate that glyphosate resistance in C. bonariensis is not the result of EPSPS mutations or overexpression, but due to a non-target-site mechanism. A large number of genes are affected by glyphosate treatment. We present a list of candidate non-target-site-resistance (NTSR) genes in fleabane for future studies into these mechanisms. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry

Item Type:Article
Business groups:Crop and Food Science
Keywords:EPSPS fleabane gene expression glyphosate, resistance transcriptome Conyza Conyza bonariensis
Subjects:Science > Botany > Genetics
Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Plant pests and diseases > Weeds, parasitic plants etc
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Pesticides
Deposited On:05 Mar 2019 02:12
Last Modified:05 Mar 2019 02:12

Repository Staff Only: item control page