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Clay nanoparticles co-deliver three antigens to promote potent immune responses against pathogenic Escherichia coli

Chen, W., Zuo, H., Rolfe, B., Schembri, M. A., Cobbold, R. N., Zhang, B., Mahony, T. J. and Xu, Z. P. (2018) Clay nanoparticles co-deliver three antigens to promote potent immune responses against pathogenic Escherichia coli. Journal of Controlled Release, 292 . pp. 196-209. ISSN 01683659 (ISSN)

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Article Link(s): https://doi.org/10.1016/j.jconrel.2018.11.008

Abstract

Currently, there are few strategies for controlling pathogenic bacteria, especially the pathotypes of Escherichia coli which are an emerging threat to public health worldwide. Here, multivalent vaccine formulations are reported for control of pathogenic E. coli. The formulations utilised clay nanoparticles, either layered double hydroxides (LDH) or hectorite (HEC), to complex with a cocktail of three recombinant antigens, intimin β (IB), proprietary antigen 1 (PAg1) and proprietary antigen 2 (PAg2). Acting as nano-adjuvants, LDH and HEC were able to stimulate strong, durable and balanced immune responses in mice. Moreover, LDH-IB-PAg1-PAg2 and HEC-IB-PAg1-PAg2 immunised mice developed potent mucosal immune responses and efficiently prevented adherence of enterohemorrhagic E. coli serotype O26 to mammalian cells. Notably, the multi-faceted immune responses elicited by the clay nanoparticle formulations were significantly higher than those induced by a QuilA formulation, without antigenic competition observed for the first time. The results of this study suggest that LDH and HEC offer considerable promise as effective multivalent vaccine carriers against important pathogens such as enteropathogenic E. coli. © 2018

Item Type:Article
Business groups:Animal Science
Keywords:Antigenic competition Enterohemorrhagic Escherichia coli Hectorite Layered double hydroxide Multivalent vaccination Antigens Escherichia coli Health risks Mammals Nanoparticles Pathogens Silver compounds Vaccines Enteropathogenic e. coli Hectorites Layered double hydroxides Mucosal immune response Pathogenic bacterium Pathogenic Escherichia coli Immune system
Subjects:Science > Microbiology
Science > Microbiology > Immunology
Animal culture > Small animal culture
Veterinary medicine > Veterinary bacteriology
Veterinary medicine > Communicable diseases of animals (General)
Deposited On:17 Jan 2019 02:32
Last Modified:17 Jan 2019 02:32

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