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Influence of omega-3 polyunsaturated fatty acids from fish oil or meal on the structure of lipid microdomains in bovine luteal cells

Plewes, M. R., Burns, P. D., Graham, P. E., Bruemmer, J. E. and Engle, T. E. (2018) Influence of omega-3 polyunsaturated fatty acids from fish oil or meal on the structure of lipid microdomains in bovine luteal cells. Animal Reproduction Science, 193 . pp. 40-57. ISSN 0378-4320

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Article Link(s): https://doi.org/10.1016/j.anireprosci.2018.03.036

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0378432017306504

Abstract

Biological membranes are composed of a lipid bilayer and proteins that form lipid microdomains. This study examined the effects of fish byproducts on lipid-protein interactions within lipid microdomains of bovine luteal cells. In Exp. 1 and 2, luteal cells were prepared from corpora lutea (CL; n = 4 to 8) collected at an abattoir. Exp. 1 was conducted to optimize ultrasonication in a detergent-free protocol for isolation of lipid microdomains. A power setting of 10 to 20% was effective in isolating lipid microdomains from bulk lipid. In Exp. 2, cells were cultured in control medium or fish oil to determine influence of fish oil on distribution of lipid microdomain markers and prostaglandin F2α (FP) receptors. Cells treated with fish oil had a smaller percentage of microdomain markers and FP receptor in microdomains (P < 0.05). In Exp. 3 and 4, cells were prepared from mid-cycle CL obtained from cows supplemented with corn gluten meal (n = 4) or fish meal (n = 4). Exp. 3 examined effects of dietary supplementation on distribution of lipid microdomain markers and FP receptor and Exp. 4 on fatty acid composition within lipid microdomains. A smaller percentage of lipid microdomain markers and FP receptor was detected in microdomains of cells collected from fish meal supplemented animals (P < 0.05). In Exp. 4, a greater percentage of omega-3 polyunsaturated fatty acids was detected in bulk lipid from fish meal supplemented cows (P < 0.05). Results show that fish byproducts influence lipid-protein interactions in lipid microdomains in bovine luteal cells.

Item Type:Article
Business groups:Animal Science
Keywords:Bovine Lipid microdomain Omega-3 polyunsaturated fatty acid Corpus luteum FP receptor G-protein-coupled receptor
Subjects:Science > Biology > Molecular Biology
Animal culture > Cattle
Animal culture > Feeds and feeding. Animal nutrition
Deposited On:16 Aug 2018 06:50
Last Modified:16 Aug 2018 06:50

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