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Stable isotope dilution assay (SIDA) and HS-SPME-GCMS quantification of key aroma volatiles for fruit and sap of Australian mango cultivars

San, Anh T. and Joyce, Daryl C. and Hofman, Peter J. and Macnish, Andrew J. and Webb, Richard I. and Matovic, Nicolas J. and Williams, Craig M. and De Voss, James J. and Wong, Siew H. and Smyth, Heather E. (2017) Stable isotope dilution assay (SIDA) and HS-SPME-GCMS quantification of key aroma volatiles for fruit and sap of Australian mango cultivars. Food Chemistry, 221 . pp. 613-619. ISSN 0308-8146

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Article Link(s): http://dx.doi.org/10.1016/j.foodchem.2016.11.130

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0308814616319793

Abstract

Reported herein is a high throughput method to quantify in a single analysis the key volatiles that contribute to the aroma of commercially significant mango cultivars grown in Australia. The method constitutes stable isotope dilution analysis (SIDA) in conjunction with headspace (HS) solid-phase microextraction (SPME) coupled with gas-chromatography mass spectrometry (GCMS). Deuterium labelled analogues of the target analytes were either purchased commercially or synthesised for use as internal standards. Seven volatiles, hexanal, 3-carene, α-terpinene, p-cymene, limonene, α-terpinolene and ethyl octanoate, were targeted. The resulting calibration functions had determination coefficients (R2) ranging from 0.93775 to 0.99741. High recovery efficiencies for spiked mango samples were also achieved. The method was applied to identify the key aroma volatile compounds produced by ‘Kensington Pride’ and ‘B74’ mango fruit and by ‘Honey Gold’ mango sap. This method represents a marked improvement over current methods for detecting and measuring concentrations of mango fruit and sap volatiles.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Gas chromatography–mass spectrometry (GC–MS) Mangifera indica Mango fruit Solid-phase microextraction (SPME) Stable-isotope dilution assay (SIDA) Volatiles
Subjects:Agriculture > Agriculture (General) > Agricultural chemistry. Agricultural chemicals
Plant culture > Food crops
Plant culture > Fruit and fruit culture
Technology > Technology (General) > Spectroscopy
Deposited On:19 Jan 2017 03:37
Last Modified:19 Jan 2017 03:37

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