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Mapping the carriage of flaA-restriction fragment length polymorphism Campylobacter genotypes on poultry carcasses through the processing chain and comparison to clinical isolates

Duffy, Lesley L. and Blackall, Patrick J. and Cobbold, Rowland N. and Fegan, Narelle (2015) Mapping the carriage of flaA-restriction fragment length polymorphism Campylobacter genotypes on poultry carcasses through the processing chain and comparison to clinical isolates. Food Microbiology, 48 . pp. 116-122.

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Article Link(s): http://dx.doi.org/10.1016/j.fm.2014.12.009

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0740002014003220

Abstract

Poultry are considered a major source for campylobacteriosis in humans. A total of 1866 Campylobacter spp. isolates collected through the poultry processing chain were typed using flaA-restriction fragment length polymorphism to measure the impact of processing on the genotypes present. Temporally related human clinical isolates (n = 497) were also typed. Isolates were obtained from whole chicken carcass rinses of chickens collected before scalding, after scalding, before immersion chilling, after immersion chilling and after packaging as well as from individual caecal samples. A total of 32 genotypes comprising at least four isolates each were recognised. Simpson's Index of Diversity (D) was calculated for each sampling site within each flock, for each flock as a whole and for the clinical isolates. From caecal collection to after packaging samples the D value did not change in two flocks, decreased in one flock and increased in the fourth flock. Dominant genotypes occurred in each flock but their constitutive percentages changed through processing. There were 23 overlapping genotypes between clinical and chicken isolates. The diversity of Campylobacter is flock dependant and may alter through processing. This study confirms that poultry are a source of campylobacteriosis in the Australian population although other sources may contribute.

Item Type:Article
Business groups:Animal Science
Keywords:Campylobacter Poultry flaA-RFLP Processing
Subjects:Science > Microbiology
Science > Microbiology > Bacteria
Animal culture > Poultry
Veterinary medicine > Diseases of special classes of animals
Deposited On:13 Jul 2015 05:19
Last Modified:13 Jul 2015 05:19

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