Login | Create Account (DAF staff only)

The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia

Babra, C. and Tiwari, J. G. and Pier, G. and Thein, T. H. and Sunagar, R. and Sundareshan, S. and Isloor, S. and Hegde, N. R. and de Wet, S. and Deighton, M. and Gibson, J. and Costantino, P. and Wetherall, J. and Mukkur, T. (2013) The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia. Folia Microbiologica, 58 (6). pp. 469-474. ISSN 0015-5632

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://dx.doi.org/10.1007/s12223-013-0232-z

Abstract

The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3-4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:coagulase-negative staphylococci capsular polysaccharides cp vaccine infection identification colonization prevalence mechanisms antibodies virulence
Subjects:Animal culture > Cattle > Dairying
Science > Microbiology
Veterinary medicine > Diseases of special classes of animals > Cattle
Deposited On:23 Jun 2014 05:22
Last Modified:23 Jun 2014 05:22

Repository Staff Only: item control page