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In planta localization and interactions of impatiens necrotic spot tospovirus proteins

Dietzgen, R. G. and Martin, K. M. and Anderson, G. and Goodin, M. M. (2012) In planta localization and interactions of impatiens necrotic spot tospovirus proteins. Journal of General Virology, 93 . pp. 2490-2495. ISSN 0022-1317

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Article Link(s): http://dx.doi.org/10.1099/vir.0.042515-0

Abstract

Impatiens necrotic spot tospovirus (INSV) is a significant pathogen of ornamentals. The tripartite negative- and ambi-sense RNA genome encodes six proteins that are involved in cytoplasmic replication, movement, assembly, insect transmission and defence. To gain insight into the associations of these viral proteins, we determined their intracellular localization and interactions in living plant cells. Nucleotide sequences encoding the nucleoprotein N, non-structural proteins NSs and NSm, and glycoproteins Gn and Gc of a Kentucky isolate of INSV were amplified by RTPCR, cloned, sequenced and transiently expressed as fusions with autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana. All proteins accumulated at the cell periphery and co-localized with an endoplasmic reticulum marker. The Gc protein fusion also localized to the nucleus. N and NSm protein self-interactions and an NSm-N interaction were observed by using bimolecular fluorescence complementation. A tospovirus NSm homotypic interaction had not been reported previously.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information: Dietzgen, Ralf G. Martin, Kathleen M. Anderson, Gavin Goodin, Michael M. National Science Foundation; Queensland International Fellowship from the Queensland Government Department of Employment, Economic Development and Innovation, Australia This work was supported by a National Science Foundation award to M. G. R. G. D. was supported by a Queensland International Fellowship from the Queensland Government Department of Employment, Economic Development and Innovation, Australia. We thank Renyuan Wang for expert technical assistance and Amy Crume for maintenance of transgenic marker plants. Soc general microbiology Reading Part 11
Keywords:virus nucleocapsid protein wilt-virus transient expression movement protein identification domains cells nsm gc
Subjects:Science > Microbiology > Virology
Plant culture
Deposited On:03 Sep 2013 23:56
Last Modified:08 Aug 2017 15:04

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