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Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation.

Brady, P. and Elizur, A. and Williams, R. and Cummins, S.F. and Knibb, W. (2012) Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation. International Journal of Biological Sciences, 8 (3). pp. 328-343.

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Publisher URL: http://www.ivyspring.com
Article URL: http://www.ncbi.nlm.nih.gov/pubmed/22355268

Abstract

In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Additionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we investigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligonucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and captive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-beta-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyperglycaemic hormone and molt-inhibiting hormone. We could not identify differentially expressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduction.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development & Innovations (DEEDI), Animal Science, The University of Queensland, University of the Sunshine Coast
Business groups:Animal Science, Crop and Food Science
Additional Information:© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (<http://creativecommons.org/licenses/by-nc-nd/3.0/>). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
Keywords:Penaeus monodon; prawn; cephalothorax; eyestalk; ovarian maturation; gene expression; microarray; shrimp Litopenaeus-vannamei; sterol carrier protein-2; molecular-cloning; inhibiting hormone; decapod crustacean; white shrimp; lipoprotein; rhodopsin; japonicus; crayfish.
Subjects:Aquaculture and Fisheries > Aquaculture
Science > Biology > Molecular Biology
Deposited On:31 May 2012 06:45
Last Modified:31 May 2012 06:45

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