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High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections.

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Tulsiani, S.M., Craig, S.B., Graham, G.C., Cobbold, R.C., Dohnt, M.F., Burns, M.A., Jansen, C.C., Leung, L.K.P., Field, H.E. and Smythe, L.D. (2010) High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections. Annals of Tropical Medicine and Parasitology, 104 (5). pp. 427-437.

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Article Link: http://dx.doi.org/10.1179/136485910X12786389891047

Organisation URL: http://www.ingentaconnect.com/content/maney/atmp

Abstract

High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.

Item Type:Article
Corporate Creators:DEEDI
Additional Information:© W. S. Maney & Son Ltd 2010
Keywords:Family leptospiraceae; SP Nov.’ serovars; relatedness; serogroups; virulence; bacteria.
Subjects:Science > Science (General)
Science > Biology > Genetics
Science > Microbiology > Bacteria
Live Archive:09 Nov 2010 07:28
Last Modified:03 Sep 2021 16:48

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