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An innovative microplate assay to facilitate the detection of antimicrobial activity in plant extracts.

Sultanbawa, Y. and Cusack, A. and Currie, M. and Davis, C. (2009) An innovative microplate assay to facilitate the detection of antimicrobial activity in plant extracts. Journal of Rapid Methods and Automation in Microbiology, 17 (4). pp. 519-534.

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Article Link(s): http://dx.doi.org/10.1111/j.1745-4581.2009.00187.x

Publisher URL: http://www3.interscience.wiley.com

Abstract

A microplate assay was modified for the detection of antimicrobial activity in plant extracts. The aim was to develop an in vitro assay that could rapidly screen plant extracts to provide quantitative data on inhibition of microbial growth. A spectrophotometric assay using a microplate with serial dilutions of the plant extract and the bacteria was developed. Two bacteria, Staphylococcus aureus and Escherichia coli, were used for this study. Essential oils, oregano (Origanum vulgare) and lemon myrtle (Backhousia citriodora), and three active components carvacrol, thymol and citral were evaluated. The reproducibility of the assay was high, with correlation coefficients (r aureus and E. coli between 0.9321 and 0.9816. Similarly, r and 0.9814. This assay could also be used to measure antimicrobial activity in plant extracts which vary in pH and color.

Item Type:Article
Corporate Creators:DEEDI
Additional Information:© The State of Queensland (through the Department of Employment, Economic Development and Innovation). © Wiley Periodicals, Inc.
Keywords:Antibacterial properties; assays; citral; colour; detection; essential oil plants; essential oils; growth; in vitro; medicinal plants; monoterpenoids; pathogens; pH; pharmacology; plant extracts; spectrophotometry; thymol; Escherichia coli; Myrtaceae; Origanum vulgare; plants; Staphylococcus aureus.
Subjects:Plant culture
Science > Microbiology
Deposited On:29 Jun 2010 04:32
Last Modified:21 Sep 2010 07:38

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