A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the great Artesian Basin of AustraliaPlumX View Altmetrics View AltmetricsTools Wynter, C., Patel, B.K.C., Bain, P., De Jersey, J., Hamilton, S. and Inkerman, P.A. (1996) A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the great Artesian Basin of Australia. FEMS Microbiology Letters, 140 (2-3). pp. 271-276. ISSN 0378-1097 Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link. Article Link: https://doi.org/10.1111/j.1574-6968.1996.tb08348.x AbstractA Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 °C and a pH optimum between 5 and 6. The half life was ~ 6.5 h at 75 °C and 2 h at 80 °C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB1 lAd was a member of the genus Thermoanaerobacter.
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