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Simplified analysis of pathogenic leptospiral serovars by random amplified polymorphic DNA fingerprinting

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Corney, B.G., Colley, J. and Graham, G.C. (1997) Simplified analysis of pathogenic leptospiral serovars by random amplified polymorphic DNA fingerprinting. Journal of Medical Microbiology, 46 (11). pp. 927-932. ISSN 0022-2615

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Article Link: https://doi.org/10.1099/00222615-46-11-927

Abstract

A rapid, simplified procedure combining random amplified polymorphic DNA (RAPD) fingerprinting of boiled cultures with high resolution agarose gel electrophoresis was used to compare strains from 46 pathogenic leptospiral serovars. The serovars were placed in eight groups on the basis of RAPD profile similarities. Groups 1–7 corresponded with the genome species Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii, L. weilii, L. kirschneri and L. meyeri. The eighth group did not correspond with a known genome species and may represent a new genome species. Primer choice determined the degree of discrimination possible between closely related serovars and genotypes. This procedure, unlike other procedures used for analysing taxonomic relationships between leptospiral serovars, does not require extensive DNA purification, polyacrylamide gel electrophoresis or autoradiography.

Item Type:Article
Subjects:Science > Biology > Genetics
Science > Microbiology
Live Archive:27 Mar 2024 02:27
Last Modified:27 Mar 2024 02:27

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