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Babesia bovis: Genome size, number of chromosomes and telomeric probe hybridisation

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Jones, S. H., Lew, A. E., Jorgensen, W. K. and Barker, S. C. (1997) Babesia bovis: Genome size, number of chromosomes and telomeric probe hybridisation. International Journal for Parasitology, 27 (12). pp. 1569-1573. ISSN 0020-7519

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Article Link: https://doi.org/10.1016/S0020-7519(97)00099-4

Abstract

Pulsed field gel electrophoresis of intact chromosomes of Babesia bovis revealed four chromosomes in the haploid genome. A telomere probe, derived from Plasmodium berghei, hybridised to eight SfiI restriction fragments of genomic B. bovis DNA digests indicating the presence of four chromosomes. A small subunit (18S) ribosomal RNA gene probe hybridised to the third chromsome only. The genome size of B. bovis is estimated to be 9.4 million base pairs. The sizes of chromosomes 1, 2, 3 and 4 are estimated to be 1.4, 2.0, 2.8 and 3.2 million base pairs, respectively.

Item Type:Article
Subjects:Science > Microbiology
Veterinary medicine > Veterinary parasitology
Veterinary medicine > Diseases of special classes of animals > Cattle
Live Archive:27 Mar 2024 01:41
Last Modified:27 Mar 2024 01:41

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