A new 3,6-dialkyl-α-pyrone produced by the heterologous expression of a PKS-NRPS hybrid enzyme derived from a Pestalotiopsis endophyteExport / Share Share this record       Export this recordPlumX View Altmetrics View AltmetricsShi, Y., Ozaki, T., Sugawara, A., Morishita, Y., Tan, Y. P., Shivas, R. G., Davis, R. A. and Asai, T. (2024) A new 3,6-dialkyl-α-pyrone produced by the heterologous expression of a PKS-NRPS hybrid enzyme derived from a Pestalotiopsis endophyte. Tetrahedron Letters, 134 . p. 154865. ISSN 0040-4039 Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link. Article Link: https://doi.org/10.1016/j.tetlet.2023.154865 Publisher URL: https://www.sciencedirect.com/science/article/pii/S0040403923005750 AbstractGenome mining of polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) hybrid enzymes independent of trans-acting enoyl reductase using our in-house fungal draft genomic library led to the discovery of a unique PKS-NRPS hybrid enzyme, PbpsA, in a Pestalotiopsis fungus. Heterologously expressed PbpsA in Aspergillus oryzae provided a new 3,6-dialkyl-α-pyrone 1. This compound was fully characterized following the interpretation of NMR, UV, IR, and MS data. From the structure, we speculated that 1 was biosynthesized by the only PKS moiety of PbpsA, and that a 3-ethyl substitution was introduced by an endogenous enzyme from A. oryzae.
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