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Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology

Candresse, T. and Lot, H. and German-Retana, S. and Krause-Sakate, R. and Thomas, J. and Souche, S. and Delaunay, T. and Lanneau, M. and Le Gall, O. (2007) Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology. Journal of General Virology, 88 (9). pp. 2605-2610.

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Article Link(s): http://dx.doi.org/10.1099/vir.0.82980-0

Publisher URL: http://vir.sgmjournals.org/

Abstract

A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science, Horticulture and Forestry Science
Additional Information:© Society for General Microbiology.
Keywords:monoclonal antibodies; (mAbs); immunological variability; Lettuce mosaic virus; (LMV); Potyvirus.
Subjects:Science > Microbiology > Virology
Plant pests and diseases
Deposited On:29 Jan 2009 00:53
Last Modified:27 Oct 2011 01:45

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