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Influence of environmental and instrumental variables on the non-invasive prediction of Brix in pineapple using near infrared spectroscopy

Guthrie, J. and Walsh, K.B. (1999) Influence of environmental and instrumental variables on the non-invasive prediction of Brix in pineapple using near infrared spectroscopy. Australian Journal of Experimental Agriculture, 39 (1). pp. 73-80.

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Article Link(s): http://dx.doi.org/10.1071/EA96127

Publisher URL: http://www.publish.csiro.au

Abstract

The Brix content of pineapple fruit can be non-invasively predicted from the second derivative of near infrared reflectance spectra. Correlations obtained using a NIRSystems 6500 spectrophotometer through multiple linear regression and modified partial least squares analyses using a post-dispersive configuration were comparable with that from a pre-dispersive configuration in terms of accuracy (e.g. coefficient of determination, R2, 0.73; standard error of cross validation, SECV, 1.01°Brix). The effective depth of sample assessed was slightly greater using the post-dispersive technique (about 20 mm for pineapple fruit), as expected in relation to the higher incident light intensity, relative to the pre-dispersive configuration. The effect of such environmental variables as temperature, humidity and external light, and instrumental variables such as the number of scans averaged to form a spectrum, were considered with respect to the accuracy and precision of the measurement of absorbance at 876 nm, as a key term in the calibration for Brix, and predicted Brix.

The application of post-dispersive near infrared technology to in-line assessment of intact fruit in a packing shed environment is discussed.

Item Type:Article
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Instrumental variables; infrared spectroscopy; Brix.
Subjects:Technology > Technology (General) > Spectroscopy > NIR (Near Infrared)
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Plant culture > Harvesting, curing, storage
Deposited On:09 Jul 2004
Last Modified:15 Mar 2011 06:54

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