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Bacteriophages that infect the cellulolytic ruminal bacterium Ruminococcus albus AR67

Klieve, A.V. and Bain, P.A. and Yokoyama, M.T. and Ouwerkerk, D. and Foster, R.J. and Turner, A.F. (2004) Bacteriophages that infect the cellulolytic ruminal bacterium Ruminococcus albus AR67. Letters in Applied Microbiology, 38 (4). pp. 333-338.

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Article Link(s): http://dx.doi.org/10.1111/j.1472-765X.2004.01493.x

Publisher URL: http://www.blackwell-synergy.com

Abstract

Aim: To isolate bacterial viruses that infect the ruminal cellulolytic bacterium Ruminococcus albus.

Methods: Four phages infecting R. albus AR67 were isolated under anaerobic conditions using the soft-agar overlay technique. The phages were characterized on morphology, solvent stability, nucleic acid type and digestion characteristics. Two phages, Ra02 and Ra04 comprised icosahedral virions with linear double-stranded DNA and appeared to belong to the family Tectiviridae. The other two phages are most likely filamentous phages with circular single-stranded DNA of the family Inoviridae.

Significance of the Study: Viruses of the families Tectiviridae and Inoviridae have not previously been isolated from rumen bacteria. The phages isolated in this study are the first phages shown to infect the cellulolytic bacteria of the rumen. This suggests that the cellulolytic populations of the rumen are subject to lytic events that may impact on the ability of these bacteria to degrade plant fibre and on the nutrition of the animal.

Item Type:Article
Additional Information:© Blackwell Publishing Ltd. Journal subscribers may be able to access the published version of this article. Use hypertext link above to access publisher’s web site
Keywords:Inoviridae; rumen; bacterial viruses; cellulose; Ruminococcus; Tectiviridae.
Subjects:Science > Microbiology > Microbial ecology
Veterinary medicine > Diseases of special classes of animals
Veterinary medicine > Veterinary bacteriology
Deposited On:26 Mar 2004
Last Modified:07 Jun 2011 06:01

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