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Differentiation of peanut seedborne potyviruses and cucumoviruses by RT-PCR

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Dietzgen, R. G., Callaghan, B., Higgins, C. M., Birch, R.G., Chen, K. and Xu, Z. (2001) Differentiation of peanut seedborne potyviruses and cucumoviruses by RT-PCR. Plant Disease, 85 (9). pp. 989-992. ISSN 0191-2917


Article Link: https://doi.org/10.1094/PDIS.2001.85.9.989


Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.

Item Type:Article
Subjects:Plant pests and diseases > Individual or types of plants or trees
Live Archive:10 Jan 2024 04:39
Last Modified:10 Jan 2024 04:39

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