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Molecular identification and characterisation of Mannheimia haemolytica

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Kayal, A., Nahar, N., Barker, L., Tran, T., Williams, M., Blackall, P. J., Turni, C. and Omaleki, L. (2024) Molecular identification and characterisation of Mannheimia haemolytica. Veterinary Microbiology, 288 . p. 109930. ISSN 0378-1135


Article Link: https://doi.org/10.1016/j.vetmic.2023.109930

Publisher URL: https://www.sciencedirect.com/science/article/pii/S0378113523002845


Mannheimia haemolytica is known as one of the major bacterial contributors to Bovine Respiratory Disease (BRD) syndrome. This study sought to establish a novel species-specific PCR to aid in identification of this key pathogen. As well, an existing multiplex PCR was used to determine the prevalence of serovars 1, 2 or 6 in Australia. Most of the 65 studied isolates originated from cattle with a total of 11 isolates from small ruminants. All problematic field isolates in the identification or serotyping PCRs were subjected to whole genome sequencing and bioinformatic analysis. The field isolates were also subjected to rep-PCR fingerprinting. A total of 59 out of the 65 tested isolates were conformed as M. haemolytica by the new species-specific PCR which is based on the rpoB gene. The confirmed M. haemolytica field isolates were assigned to serovars 1 (24 isolates), 2 (seven isolates) and 6 (26 isolates) while two of the isolates were negative in the serotyping PCR. The two non-typeable isolates were assigned to serovar 7 and 14 following whole genome sequencing and bioinformatic analysis. The rep-PCR typing resulted in five major clusters with serovars 1 and 6 often within the same cluster. The M. haemolytica-specific PCR developed in this work was species specific and should be a valuable support for frontline diagnostic laboratories. The serotyping results support the relative importance of serovars 1 and 6 in bovine respiratory disease.

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Biosecurity Queensland
Keywords:serovar Bovine respiratory disorder BRD whole genome sequencing feedlot cattle
Subjects:Veterinary medicine > Veterinary microbiology
Veterinary medicine > Veterinary bacteriology
Veterinary medicine > Diseases of special classes of animals
Veterinary medicine > Diseases of special classes of animals > Cattle
Live Archive:11 Dec 2023 02:18
Last Modified:29 Feb 2024 03:19

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