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Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis

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Singh, D.P., Filardo, F. F., Storey, R., Jermakow, A.M., Yamaguchi, S. and Swain, S.M. (2010) Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis. Physiologia Plantarum, 138 (1). pp. 74-90. ISSN 00319317 (ISSN)

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Article Link: https://doi.org/10.1111/j.1399-3054.2009.01289.x


We have examined the role of gibberellins (GAs) in plant development by expression of the pea GA 2-oxidase2 (PsGA2ox2) cDNA, which encodes a GA inactivating enzyme, under the control of the MEDEA (MEA) promoter. Expression of MEA:PsGA2ox2 in Arabidopsis caused seed abortion, demonstrating that active GAs in the endosperm are essential for normal seed development. MEA:PsGA2ox2 plants had reduced ovule number per ovary and exhibited defects in phyllotaxy and leaf morphology which were partly suppressed by GA treatment. The leaf architecture and phyllotaxy defects of MEA:PsGA2ox2 plants were also restored by sly1-d which reduces DELLA protein stability to increase GA response. MEA:PsGA2ox2 seedlings had increased expression of the KNOTTED1-like homeobox (KNOX) genes, BP, KNAT2 and KNAT6, which are known to control plant architecture. The expression of KNOX genes is also altered in wild-type plants treated with GA. These results support the conclusion that GAs can suppress the effects of elevated KNOX gene expression, and raise the possibility that localized changes in GA levels caused by PsGA2ox2 alter the expression of KNOX genes to modify plant architecture. © 2009 Physiologia Plantarum.

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Crop and Food Science
Keywords:gibberellin gibberellin 2 dioxygenase gibberellin 2-dioxygenase homeodomain protein Knox1 protein, plant mixed function oxidase vegetable protein Arabidopsis article enzymology gene expression regulation genetics growth, development and aging metabolism pea plant leaf plant seed promoter region transgenic plant ultrastructure Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gibberellins Homeodomain Proteins Mixed Function Oxygenases Peas Plant Leaves Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic Seeds Pisum sativum
Subjects:Science > Botany > Plant anatomy
Science > Botany > Genetics
Plant culture
Live Archive:13 Apr 2023 03:55
Last Modified:13 Apr 2023 03:56

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