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Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia

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Thomas, J. E. and Dietzgen, R. G. (1991) Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. Journal of General Virology, 72 (2). pp. 217-224. ISSN 1465-2099

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Article Link(s): https://doi.org/10.1099/0022-1317-72-2-217

Publisher URL: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-2-217

Abstract

Isometric virus-like particles, 18 nm in diameter, have been isolated from banana (Musa spp.) affected by bunchy top disease in Australia. Banana bunchy top disease-associated virus-like particles (BBTV) banded as a single component with buoyant density of 1.28 to 1.29 g/ml in Cs2SO4 and sedimented at about 46S in isokinetic sucrose density gradients. The A 260/A 280 of purified preparations was about 1.33. A single coat protein of M r 20500 identified with antibodies to BBTV particles from Australia. Single-stranded DNA of about 1 kb as well as ssRNA smaller than 0.45 kb was also associated with the particles. A polyclonal antiserum to BBTV, suitable for use in ELISA, was prepared. Stability and antigenicity of purified BBTV was impaired by storage at pH ≥ 8.5 and freezing at -20 °C without protectants. BBTV was detected by double antibody sandwich-ELISA with monoclonal and polyclonal antibodies, in field-infected banana plants, single aphids from an infective colony, and in experimentally aphid-inoculated banana plants. After transmission of BBTV particles by aphids from a banana bunchy top disease-affected to an uninfected banana plant, the disease was induced and BBTV was detected by ELISA in symptomatic leaves only. BBTV isolates from Australia, Taiwan, People’s Republic of China, Tonga, Western Samoa and Hawaii were found to be serologically related, which suggests a common aetiology for the disease.

Item Type:Article
Business groups:Horticulture and Forestry Science
Subjects:Plant culture > Fruit and fruit culture
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Plant pests and diseases
Plant pests and diseases > Individual or types of plants or trees > Bananas
Plant pests and diseases > Plant pathology
Deposited On:09 Jan 2023 03:20
Last Modified:09 Jan 2023 03:20

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