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Exploration of RNA-Seq data to identify a potential pathogen of the leaf-mining moth, Stomphastis thraustica (Meyrick, 1908) (Lepidoptera: Gracillariidae)

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Etebari, K., Taylor, D. B. J., Rahman, M. M., Dhileepan, K., Furlong, M. J. and Asgari, S. (2022) Exploration of RNA-Seq data to identify a potential pathogen of the leaf-mining moth, Stomphastis thraustica (Meyrick, 1908) (Lepidoptera: Gracillariidae). Data in Brief, 40 . p. 107708. ISSN 2352-3409

[img] Microsoft Excel (Supplementary Table S1. The outcome of BLASTx search for 8,926 contigs from transcriptomic de novo assembly.)

Article Link: https://doi.org/10.1016/j.dib.2021.107708

Publisher URL: https://www.sciencedirect.com/science/article/pii/S2352340921009835


The leaf-mining moth, Stomphastis thraustica (Meyrick, 1908) was imported to Australia as a potential biological control agent of an exotic weed, bellyache bush (Jatropha gossypiifolia), from Peru. The insect colony has been maintained in the quarantine facility for over eight years but recently, significant mortality was observed in the culture. The larvae demonstrated swollen intersegments with a fragile integument. The infected larvae are cloudy muted green or yellowish whereas a healthy late instar larva is a vivid green. They slowly dehydrate and eventually die, at which point the larval body becomes rubbery and turns to black. We used next generation sequencing to identify the cause of mortality in the insects. Total RNA was extracted from 20 larvae in two cohorts, one with and one without apparent symptoms of disease, for deep sequencing on NovaSeq platform after eukaryote ribosomal RNA depletion. We identified several non-insect sequences belonging to viruses, bacteria, and fungi, but none of those showed significant abundance or enrichment in the infected dataset. The sequences related to a unicellular yeast, Saccharomyces cerevisiae, and they were among the highly expressed non-insect contigs; more than 5% of reads in both libraries mapped to the genome of this opportunistic microorganism.

Item Type:Article
Business groups:Biosecurity Queensland
Additional Information:Open access
Keywords:Insect transcriptome Insect pathology Yeast Bellyache bush Weed biological control Insect viruses
Subjects:Science > Entomology
Science > Invasive Species > Plants > Biological control
Science > Invasive Species > Plants > Weed ecology
Plant pests and diseases
Plant pests and diseases > Weeds, parasitic plants etc
Plant pests and diseases > Plant pathology
Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Live Archive:13 Dec 2021 03:31
Last Modified:18 Jan 2022 05:50

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