Login | Request Account (DAF staff only)

Establishment, Immortalisation and Characterisation of Pteropid Bat Cell Lines

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Crameri, G., Todd, S., Grimley, S., McEachern, J. A., Marsh, G. A., Smith, C., Tachedjian, M., De Jong, C., Virtue, E. R., Yu, M., Bulach, D., Liu, J.-P., Michalski, W. P., Middleton, D., Field, H. E. and Wang, L.-F. (2009) Establishment, Immortalisation and Characterisation of Pteropid Bat Cell Lines. PLOS ONE, 4 (12). e8266.

[img]
Preview
PDF
1MB

Article Link: https://doi.org/10.1371/journal.pone.0008266

Abstract

Background Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions. Methodology/Findings Black flying foxes (Pteropus alecto) were captured from the wild and transported live to the laboratory for primary cell culture preparation using a variety of different methods and culture media. Primary cells were successfully cultured from 20 different organs. Cell immortalisation can occur spontaneously, however we used a retroviral system to immortalise cells via the transfer and stable production of the Simian virus 40 Large T antigen and the human telomerase reverse transcriptase protein. Initial infection experiments with both cloned and uncloned cell lines using Hendra and Nipah viruses demonstrated varying degrees of infection efficiency between the different cell lines, although it was possible to infect cells in all tissue types. Conclusions/Significance The approaches developed and optimised in this study should be applicable to bats of other species. We are in the process of generating further cell lines from a number of different bat species using the methodology established in this study.

Item Type:Article
Additional Information:Open access PDF attached
Live Archive:29 Sep 2020 00:07
Last Modified:03 Sep 2021 16:46

Repository Staff Only: item control page

Downloads

Downloads per month over past year

View more statistics