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Pasteurella multocida detection by 5' Taq nuclease assay: a new tool for use in diagnosing fowl cholera

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Corney, B., Diallo, I., Wright, L., Hewitson, G., De Jong, A., Burrell, P., Duffy, P., Stephens, C.P., Rodwell, B., Boyle, D.B. and Blackall, P.J. (2007) Pasteurella multocida detection by 5' Taq nuclease assay: a new tool for use in diagnosing fowl cholera. Journal of Microbiological Methods, 69 (2). pp. 376-380.


Article Link: http://dx.doi.org/10.1016/j.mimet.2007.01.014

Publisher URL: http://www.elsevier.com


A 5′ Taq nuclease assay utilising minor groove binder technology and targeting the 16S rRNA gene was designed to detect Pasteurella multocida (the causative agent of fowl cholera) in swabs collected from poultry. The assay was first evaluated using pure cultures. The assay correctly identified four P. multocida taxonomic type strains, 18 P. multocida serovar reference strains and 40 Australian field isolates (17 from poultry, 11 from pigs and 12 from cattle). Representatives of nine other Pasteurella species, 26 other bacterial species (18 being members of the family Pasteurellaceae) and four poultry virus isolates did not react in the assay. The assay detected a minimum of approximately 10 cfu of P. multocida per reaction. Of 79 poultry swabs submitted to the laboratory for routine bacteriological culture, 17 were positive in the 5′ Taq nuclease assay, but only 10 were positive by culture. The other 62 swabs were negative for P. multocida by both 5′ Taq nuclease assay and culture. The assay is suitable for use in diagnosing fowl cholera, is more rapid than bacteriological culture, and may also have application in diagnosing P. multocida infections in cattle and pigs.

Item Type:Article
Corporate Creators:Biosecurity Queensland, Animal Science
Additional Information:Author version © Queensland Department of Primary Industries and Fisheries. Reproduced in accordance with the copyright policy of the publisher. © Elsevier. Access to published version may be available via Publisher’s website.
Keywords:Pasteurella multocida; fowl cholera; PCR; 5′ Taq nuclease assay; minor groove binder.
Subjects:Veterinary medicine > Diseases of special classes of animals > Avian
Veterinary medicine > Veterinary bacteriology
Live Archive:25 Feb 2008
Last Modified:03 Sep 2021 16:47

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