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Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks

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Kamau, J., De Vos, A. J., Playford, M., Salim, B., Kinyanjui, P. and Sugimoto, C. (2011) Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks. Parasites and Vectors, 4 (1).

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Article Link: http://doi.org/10.1186/1756-3305-4-22

Publisher URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-79951688744&partnerID=40&md5=5ebc962268c48e20534b8c7278b44b4bhttp://download.springer.com/static/pdf/546/art%253A10.1186%252F1756-3305-4-22.pdf?originUrl=http%3A%2F%2Fparasitesandvectors.biomedcentral.co


Theileria parasites cause a benign infection of cattle in parts of Australia where they are endemic, but have, in recent years, been suspected of being responsible for a number of outbreaks of disease in cattle near the coast of New South Wales. The objective of this study was to identify and characterize the species of Theileria in cattle on six farms in New South Wales where disease outbreaks have occurred, and compare with Theileria from three disease-free farms in Queensland that is endemic for Theileria. Special reference was made to sub-typing of T. orientalis by type-specific PCR and sequencing of the small subunit (SSU) rRNA gene, and sequence analysis of the gene encoding a polymorphic merozoite/piroplasm surface protein (MPSP) that may be under immune selection. Nucleotide sequencing of SSU rRNA and MPSP genes revealed the presence of four Theileria genotypes: T. orientalis (buffeli), T. orientalis (ikeda), T. orientalis (chitose) and T. orientalis type 4 (MPSP) or type C (SSU rRNA). The majority of animals showed mixed infections while a few showed single infection. When MPSP nucleotide sequences were translated into amino acids, base transition did not change amino acid composition of the protein product, suggesting possible silent polymorphism. The occurrence of ikeda and type 4 (type C) previously not reported to occur and silent mutation is thought to have enhanced parasite evasion of the host immune response causing the outbreak. © 2011 Kamau et al; licensee BioMed Central Ltd.

Item Type:Article
Keywords:membrane protein merozoite surface protein 1 merozoite surface protein 2 piroplasm surface protein small subunit ribosomal RNA unclassified drug major piroplasm surface protein, Theileria parasite antigen protozoal DNA protozoal protein protozoal RNA ribosome DNA RNA 18S amino acid composition animal experiment animal model article Australia cattle endemic disease epidemic gene mutation genetic code genotype immune response merozoite mixed infection nonhuman nucleotide sequence polymerase chain reaction protein polymorphism sequence analysis Theileria Theileria orientalis theileriosis animal chemistry classification cluster analysis DNA sequence genetics isolation and purification molecular genetics molecular typing parasitology phylogeny RNA gene Animalia Bos Animals Antigens, Protozoan Disease Outbreaks DNA, Protozoan DNA, Ribosomal Genes, rRNA Molecular Sequence Data New South Wales Protozoan Proteins Queensland RNA, Protozoan RNA, Ribosomal, 18S Sequence Analysis, DNA Theileriasis
Subjects:Animal culture > Cattle
Veterinary medicine > Veterinary parasitology
Live Archive:28 Mar 2019 01:08
Last Modified:03 Sep 2021 16:45

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