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Characterisation of Rph24: A gene conferring adult plant resistance to Puccinia hordei in barley

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Ziems, L. A., Hickey, L. T., Platz, G. J., Franckowiak, J. D., Dracatos, P. M., Singh, D. and Park, R. F. (2017) Characterisation of Rph24: A gene conferring adult plant resistance to Puccinia hordei in barley. Phytopathology, 107 (7). pp. 834-841. ISSN 0031-949X

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Article Link: http://dx.doi.org/10.1094/PHYTO-08-16-0295-R

Publisher URL: http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-08-16-0295-R


We identified Rph24 as a locus in barley (Hordeum vulgare L.) controlling adult plant resistance (APR) to leaf rust, caused by Puccinia hordei. The locus was previously reported as a quantitative trait locus in barley line ND24260-1 and named qRphND. We crossed ND24260-1 to the leaf rust susceptible standard Gus and determined inheritance patterns in the progeny. For the comparative marker frequency analysis (MFA), ‘resistant’ and ‘susceptible’ tails of the F2 were genotyped with Diversity Arrays Technology genotyping-by-sequencing (DArT-Seq) markers. The Rph24 locus was positioned at 55.5 cM on chromosome 6H on the DArT-Seq consensus map. Evaluation of F2:3 families confirmed that a single locus from ND24260-1 conferred partial resistance. The haploblock strongly associated with the Rph24 locus was used to estimate the allele frequency in a collection of 282 international barley cultivars. Rph24 was frequently paired with APR locus Rph20 in cultivars displaying high levels of APR to leaf rust. The markers identified in this study for Rph24 should be useful for marker-assisted selection.

Item Type:Article
Business groups:Crop and Food Science
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Barley
Plant pests and diseases
Plant pests and diseases > Plant pathology
Live Archive:10 Jul 2017 02:31
Last Modified:03 Sep 2021 16:51

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