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New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia

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Mohr, P. G., Moody, N. J. G., Hoad, J., Williams, L. M., Bowater, R. O., Cummins, D. M., Cowley, J. A. and Crane, M. S. (2015) New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia. Diseases of Aquatic Organisms, 115 (3). pp. 263-268.

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Article Link: http://dx.doi.org/10.3354/dao02894

Publisher URL: http://www.int-res.com/abstracts/dao/v115/n3/p263-268/


ABSTRACT: In 2012, giant tiger shrimp <i>Penaeus monodon</i> originally sourced from Joseph Bonaparte Gulf in northern Australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a Queensland hatchery. Nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the OIE YHV Protocol 2 RT-PCR designed to differentiate yellow head virus (YHV1) from gill-associated virus (GAV, synonymous with YHV2) and the OIE YHV Protocol 3 RT-nested PCR designed for consensus detection of YHV genotypes. Sequence analysis of the 794 bp (Protocol 2) and 359 bp (Protocol 3) amplicons from 2 distinct regions of ORF1b showed that the yellow-head-complex virus detected was novel when compared with Genotypes 1 to 6. Nucleotide identity on the Protocol 2 and Protocol 3 ORF1b sequences was highest with the highly pathogenic YHV1 genotype (81 and 87%, respectively) that emerged in <i>P. monodon</i> in Thailand and lower with GAV (78 and 82%, respectively) that is enzootic to <i>P. monodon</i> inhabiting eastern Australia. Comparison of a longer (725 bp) ORF1b sequence, spanning the Protocol 3 region and amplified using a modified YH30/31 RT-nPCR, provided further phylogenetic evidence for the virus being distinct from the 6 described YHV genotypes. The virus represents a unique seventh YHV genotype (YHV7). Despite the mortalities observed, the role of YHV7 remains unknown.

Item Type:Article
Business groups:Biosecurity Queensland
Additional Information:10.3354/dao02894
Live Archive:09 Feb 2016 01:34
Last Modified:03 Sep 2021 16:50

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