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Cytorhabdovirus phosphoprotein shows RNA silencing suppressor activity in plants, but not in insect cells

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Mann, K. S., Johnson, K. N. and Dietzgen, R. G. (2015) Cytorhabdovirus phosphoprotein shows RNA silencing suppressor activity in plants, but not in insect cells. Virology, 476 . p. 413. ISSN 00426822

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Article Link: http://dx.doi.org/10.1016/j.virol.2014.12.023

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0042682214005728


RNA silencing in plants and insects provides an antiviral defense and as a countermeasure most viruses encode RNA silencing suppressors (RSS). For the family Rhabdoviridae, no detailed functional RSS studies have been reported in plant hosts and insect vectors. In agroinfiltrated Nicotiana benthamiana leaves we show for the first time for a cytorhabdovirus, lettuce necrotic yellows virus (LNYV), that one of the nucleocapsid core proteins, phosphoprotein (P) has relatively weak local RSS activity and delays systemic silencing of a GFP reporter. Analysis of GFP small RNAs indicated that the P protein did not prevent siRNA accumulation. To explore RSS activity in insects, we used a Flock House virus replicon system in Drosophila S2 cells. In contrast to the plant host, LNYV P protein did not exhibit RSS activity in the insect cells. Taken together our results suggest that P protein may target plant-specific components of RNA silencing post siRNA biogenesis.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Agroinfiltration Drosophila S2 cells Flock House virus Green fluorescent protein Lettuce necrotic yellows virus RNA silencing suppression Viral protein expression RNA RNA silencing suppressor protein small interfering RNA unclassified drug virus phosphoprotein virus protein animal cell Article controlled study Drosophila gene silencing insect cell Lettuce necrotic yellow virus Nicotiana benthamiana nonhuman plant cell replicon reporter gene Rhabdoviridae Cytorhabdovirus Hexapoda
Subjects:Plant culture > Food crops
Science > Botany > Genetics
Plant pests and diseases
Live Archive:18 Mar 2015 01:43
Last Modified:03 Sep 2021 16:50

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