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Australian bat lyssavirus infection in two horses

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Shinwari, M. W., Annand, E. J., Driver, L., Warrilow, D., Harrower, B., Allcock, R. J. N., Pukallus, D., Harper, J., Bingham, J., Kung, N. and Diallo, I. S. (2014) Australian bat lyssavirus infection in two horses. Veterinary Microbiology, 173 (3–4). pp. 224-231. ISSN 0378-1135

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Article Link: http://dx.doi.org/10.1016/j.vetmic.2014.07.029

Publisher URL: http://www.sciencedirect.com/science/article/pii/S037811351400371X

Abstract

In May 2013, the first cases of Australian bat lyssavirus infections in domestic animals were identified in Australia. Two horses (filly-H1 and gelding-H2) were infected with the Yellow-bellied sheathtail bat (YBST) variant of Australian bat lyssavirus (ABLV). The horses presented with neurological signs, pyrexia and progressing ataxia. Intra-cytoplasmic inclusion bodies (Negri bodies) were detected in some Purkinje neurons in haematoxylin and eosin (H&E) stained sections from the brain of one of the two infected horses (H2) by histological examination. A morphological diagnosis of sub-acute moderate non-suppurative, predominantly angiocentric, meningo-encephalomyelitis of viral aetiology was made. The presumptive diagnosis of ABLV infection was confirmed by the positive testing of the affected brain tissue from (H2) in a range of laboratory tests including fluorescent antibody test (FAT) and real-time PCR targeting the nucleocapsid (N) gene. Retrospective testing of the oral swab from (H1) in the real-time PCR also returned a positive result. The FAT and immunohistochemistry (IHC) revealed an abundance of ABLV antigen throughout the examined brain sections. ABLV was isolated from the brain (H2) and oral swab/saliva (H1) in the neuroblastoma cell line (MNA). Alignment of the genome sequence revealed a 97.7% identity with the YBST ABLV strain.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Australian bat lyssavirus Equine non-suppurative meningoencephalomyelitis Negri bodies Yellow-bellied sheathtail bat
Subjects:Veterinary medicine > Diseases of special classes of animals > Horses
Veterinary medicine > Veterinary virology
Live Archive:21 Jan 2015 03:56
Last Modified:03 Sep 2021 16:50

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