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Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing

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Penton, C. R., Gupta, V. V. S. R., Tiedje, J. M., Neate, S. M., Ophel-Keller, K., Gillings, M., Harvey, P., Pham, A. and Roget, D. K. (2014) Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing. PLoS ONE, 9 (4). ISSN 19326203

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Article Link: http://dx.doi.org/10.1371/journal.pone.0093893

Abstract

Natural biological suppression of soil-borne diseases is a function of the activity and composition of soil microbial communities. Soil microbe and phytopathogen interactions can occur prior to crop sowing and/or in the rhizosphere, subsequently influencing both plant growth and productivity. Research on suppressive microbial communities has concentrated on bacteria although fungi can also influence soil-borne disease. Fungi were analyzed in co-located soils 'suppressive' or 'non-suppressive' for disease caused by Rhizoctonia solani AG 8 at two sites in South Australia using 454 pyrosequencing targeting the fungal 28S LSU rRNA gene. DNA was extracted from a minimum of 125 g of soil per replicate to reduce the micro-scale community variability, and from soil samples taken at sowing and from the rhizosphere at 7 weeks to cover the peak Rhizoctonia infection period. A total of ∼994,000 reads were classified into 917 genera covering 54% of the RDP Fungal Classifier database, a high diversity for an alkaline, low organic matter soil. Statistical analyses and community ordinations revealed significant differences in fungal community composition between suppressive and non-suppressive soil and between soil type/location. The majority of differences associated with suppressive soils were attributed to less than 40 genera including a number of endophytic species with plant pathogen suppression potentials and mycoparasites such as Xylaria spp. Non-suppressive soils were dominated by Alternaria , Gibberella and Penicillum. Pyrosequencing generated a detailed description of fungal community structure and identified candidate taxa that may influence pathogen-plant interactions in stable disease suppression. © 2014 Penton et al.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Open access PDF attached
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Soil disinfection
Plant pests and diseases > Plant pathology
Agriculture > Agriculture (General) > Soils. Soil science
Live Archive:19 Aug 2014 01:58
Last Modified:03 Sep 2021 16:44

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