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Mapping of genomic regions associated with net form of netblotch resistance in barley

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Raman, H., Platz, G.J., Chalmers, K.J., Raman, R., Read, B.J., Barr, A.R. and Moody, D.B. (2003) Mapping of genomic regions associated with net form of netblotch resistance in barley. Australian Journal of Agricultural Research, 54 (12). pp. 1359-1367.

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Article Link: http://dx.doi.org/10.1071/AR03026

Publisher URL: http://www.publish.csiro.au

Abstract

Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9–17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population.

In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science
Business groups:Crop and Food Science
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Pyrenophora teres f. teres; QTL; seedling resistance; validation.
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Plant pests and diseases > Individual or types of plants or trees > Barley
Live Archive:08 Jun 2004
Last Modified:03 Sep 2021 16:43

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