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Maternal and Paternal Genomes Differentially Affect Myofibre Characteristics and Muscle Weights of Bovine Fetuses at Midgestation

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Xiang, R.D., Ghanipoor-Samami, M., Johns, W.H., Eindorf, T., Rutley, D.L., Kruk, Z..A., Fitzsimmons, C.J., Thomsen, D.A., Roberts, C.T., Burns, B. M., Anderson, G. I., Greenwood, P.L. and Hiendleder, S. (2013) Maternal and Paternal Genomes Differentially Affect Myofibre Characteristics and Muscle Weights of Bovine Fetuses at Midgestation. Plos One, 8 (1). ISSN 1932-6203


Article Link: http://dx.doi.org/10.1371/journal.pone.0053402


Postnatal myofibre characteristics and muscle mass are largely determined during fetal development and may be significantly affected by epigenetic parent-of-origin effects. However, data on such effects in prenatal muscle development that could help understand unexplained variation in postnatal muscle traits are lacking. In a bovine model we studied effects of distinct maternal and paternal genomes, fetal sex, and non-genetic maternal effects on fetal myofibre characteristics and muscle mass. Data from 73 fetuses (Day153, 54% term) of four genetic groups with purebred and reciprocal cross Angus and Brahman genetics were analyzed using general linear models. Parental genomes explained the greatest proportion of variation in myofibre size of Musculus semitendinosus (80-96%) and in absolute and relative weights of M. supraspinatus, M. longissimus dorsi, M. quadriceps femoris and M. semimembranosus (82-89% and 56-93%, respectively). Paternal genome in interaction with maternal genome (P<0.05) explained most genetic variation in cross sectional area (CSA) of fast myotubes (68%), while maternal genome alone explained most genetic variation in CSA of fast myofibres (93%, P<0.01). Furthermore, maternal genome independently (M. semimembranosus, 88%, P<0.0001) or in combination (M. supraspinatus, 82%; M. longissimus dorsi, 93%; M. quadriceps femoris, 86%) with nested maternal weight effect (5-6%, P<0.05), was the predominant source of variation for absolute muscle weights. Effects of paternal genome on muscle mass decreased from thoracic to pelvic limb and accounted for all (M. supraspinatus, 97%, P<0.0001) or most (M. longissimus dorsi, 69%, P<0.0001; M. quadriceps femoris, 54%, P<0.001) genetic variation in relative weights. An interaction between maternal and paternal genomes (P<0.01) and effects of maternal weight (P<0.05) on expression of H19, a master regulator of an imprinted gene network, and negative correlations between H19 expression and fetal muscle mass (P<0.001), suggested imprinted genes and miRNA interference as mechanisms for differential effects of maternal and paternal genomes on fetal muscle.

Item Type:Article
Business groups:Animal Science
Additional Information:Xiang, Ruidong Ghanipoor-Samami, Mani Johns, William H. Eindorf, Tanja Rutley, David L. Kruk, Zbigniew A. Fitzsimmons, Carolyn J. Thomsen, Dana A. Roberts, Claire T. Burns, Brian M. Anderson, Gail I. Greenwood, Paul L. Hiendleder, Stefan JS Davies Bequest; Queensland Government through the Department of Agriculture, Fisheries and Forestry's Reinvestment Fund; China Scholarship Council; University of Adelaide; Iranian Ministry of Science, Research Technology This work was funded by the JS Davies Bequest with support from the Queensland Government through the Department of Agriculture, Fisheries and Forestry's Reinvestment Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.RX is a recipient of China scholarship and Adelaide University China Fee Scholarship provided by China Scholarship Council and The University of Adelaide. MG-S is recipient of a PhD scholarship from the Iranian Ministry of Science, Research & Technology. SH is a JS Davies Fellow. CTR is a NHMRC Senior Research Fellow (APP1020749). We would like to thank Mick Deland, John Cooper and Struan Research Centre farm staff (SARDI) for animal management, Sarah Truran and David Lines for assistance in fetus collection and Lin Lin for help with figures. Public library science San francisco
Keywords:meat quality traits human skeletal-muscle bos-indicus carcass traits noncoding rna fiber-type h19 gene phenotypic characterization reciprocal differences polar overdominance
Subjects:Animal culture > Cattle
Live Archive:19 Nov 2013 04:25
Last Modified:20 Jan 2023 05:32

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