Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses

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Gambley, C., Geering, A.D.W. and Thomas, J. E. (2009) Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses. Journal of Virological Methods, 155 (2). pp. 187-192.

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Article Link: http://dx.doi.org/10.1016/j.jviromet.2008.10.006

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Abstract

A semi-automated, immunomagneticcapture-reverse transcription PCR(IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available.

Item Type:	Article
Business groups:	Crop and Food Science, Horticulture and Forestry Science
Additional Information:	© Crown Copyright.
Keywords:	Semi-automation; mealybug wilt disease; pineapple mealybug wilt-associated virus; RT-PCR; Hepatitis A virus; polymerase chain reaction (PCR); hybridization RT-PCR; genetic diversity; immunocapture; Australia; disease; banana.
Subjects:	Science > Science (General) Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple Plant pests and diseases > Plant pathology Science > Biology > Genetics
Live Archive:	16 Apr 2009 02:45
Last Modified:	12 Sep 2022 06:49

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