Login | Request Account (DAF staff only)

Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Weed Plant Species

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Chen, A., Morrison, S., Gregson, A., Le, D. P., Urquhart, A. S., Smith, L. J., Aitken, E. A. B. and Gardiner, D. M. (2024) Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Weed Plant Species. Pathogens, 13 (6). p. 442. ISSN 2076-0817

[img] Archive (ZIP) (Supplementary material)

Article Link: https://doi.org/10.3390/pathogens13060442

Publisher URL: https://www.mdpi.com/2076-0817/13/6/442


Verticillium wilt is a soil-borne disease caused by distinct vegetative compatibility groups (VCG) of the fungus Verticillium dahliae. Defoliating (VCG 1A) and non-defoliating (VCG 2A) pathotypes of V. dahliae have contributed to yield losses of cotton production in Australia. To study the virulence and the infection process of V. dahliae on cotton, two isolates, one representing each VCG, have been transformed with fluorescent protein genes. The transformants maintained their ability to infect the host, and both strains were observed to move through the plant vasculature to induce wilt symptoms. Furthermore, virulence testing suggests that the cotton V. dahliae strains can endophytically colonise common weed plant species found in the Australian landscape, and that is contrasted by their ability to infect and colonise native tobacco plants. The fluorescently labelled strains of V. dahliae not only allowed us to gain a thorough understanding of the infection process but also provided a method to rapidly identify recovered isolates from host colonisation studies.

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Crop and Food Science
Keywords:alternative weed plant host; defoliating and non-defoliating pathotypes; green fluorescent protein; mCherry red fluorescent protein; Nicotina benthamiana; plant host and pathogen interactions; reporter genes; vegetative compatibility groups 1A and 2A
Subjects:Plant culture > Field crops > Textile and fibre plants
Plant pests and diseases
Plant pests and diseases > Weeds, parasitic plants etc
Plant pests and diseases > Plant pathology
Live Archive:17 Jun 2024 05:59
Last Modified:17 Jun 2024 05:59

Repository Staff Only: item control page


Downloads per month over past year

View more statistics