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The development of multiplex PCR assays for the rapid identification of multiple Saccostrea species, and their practical applications in restoration and aquaculture

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Richardson, M. A., Nenadic, N., Wingfield, M. and McDougall, C. (2024) The development of multiplex PCR assays for the rapid identification of multiple Saccostrea species, and their practical applications in restoration and aquaculture. BMC Ecology and Evolution, 24 (1). p. 67. ISSN 2730-7182

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Article Link: https://doi.org/10.1186/s12862-024-02250-1

Abstract

The ecology and biology of oysters (Ostreidae) across the tropics is poorly understood. Morphological plasticity and shared characteristics among oysters have resulted in the misidentification of species, creating challenges for understanding basic species-specific biological information that is required for restoration and aquaculture. Genetic barcoding has proven essential for accurate species identification and understanding species geographic ranges. To reduce the costs of molecular species identification we developed multiplex assays using the cytochrome c oxidase subunit I (COI or cox1) barcoding gene for the rapid identification of five species of oysters within the genus Saccostrea that are commonly found in Queensland, Australia: Saccostrea glomerata, Saccostrea lineage B, Saccostrea lineage F, Saccostrea lineage G, and Saccostrea spathulata (lineage J).

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Animal Science
Keywords:Saccostrea Aquaculture Restoration Oyster Oyster reef Oyster reef restoration Molecular Species-specific PCR Tropical oysters
Subjects:Aquaculture and Fisheries > Aquaculture
Aquaculture and Fisheries > Aquaculture > Shellfish culture
Agriculture > By region or country > Australia
Live Archive:06 Jun 2024 06:14
Last Modified:06 Jun 2024 06:14

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