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Babesia bovis: Biosynthesis and localisation of the 12D3 antigen in bovine erythrocytes

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Harper, G.S., Hibbs, A.R., East, I.J., Waltisbuhl, D.J., Jorgensen, W.K. and Riddles, P.W. (1996) Babesia bovis: Biosynthesis and localisation of the 12D3 antigen in bovine erythrocytes. International Journal for Parasitology, 26 (11). pp. 1255-1262. ISSN 0020-7519

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Article Link: https://doi.org/10.1016/S0020-7519(96)00105-1


The 12D3 antigen of Babesia bovis was found to be synthesised rapidly in cultured parasites, and localised to both the apical complex of the merozoite and the cytoplasm of the parasitised erythrocyte. Amino-terminal sequencing suggested that the nascent protein had been processed and differences between the predicted and measured molecular weights suggested post-translational modification. The major proportion of 12D3 appeared in the soluble compartment of the parasitised erythrocytes with a molecular weight consistent with no further processing. A significant proportion of the protein required extraction by sodium carbonate, suggesting association with membranous components. The timing of release of soluble 12D3 was coincident with haemoglobin release and this probably reflects a non-specific lysis of the erythrocyte. Synthesis of recombinant BV12D3 was achieved in baculovirus-infected SF9 insect epithelial cells. The product was of the same molecular weight as the native 12D3 and polyclonal antibodies raised against the recombinant protein reacted with both the recombinant and native forms of the antigen.

Item Type:Article
Keywords:Babesia bovis; haemoprotozoa; biosynthesis; synthetic products; recombinant antigens
Subjects:Veterinary medicine > Veterinary pathology
Veterinary medicine > Veterinary parasitology
Veterinary medicine > Diseases of special classes of animals > Cattle
Live Archive:10 Apr 2024 00:51
Last Modified:10 Apr 2024 00:51

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