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Use of optical density as a measure of Claviceps africana conidial suspension concentration

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Herde, D.J., Ryley, M.J., Foster, S.D., Galea, V.J., Henzell, R.G. and Jordan, D.R. (2006) Use of optical density as a measure of Claviceps africana conidial suspension concentration. Australasian Plant Pathology, 35 (1). pp. 77-80.

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Article Link: http://dx.doi.org/10.1071/AP05093

Publisher URL: http://www.publish.csiro.au/


Sorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science, Plant Science
Business groups:Crop and Food Science
Additional Information:© Australasian Plant Pathology Society. © CSIRO Publishing.
Keywords:Colorimeter; Inoculum concentration; light transmittance; Sorghum bicolor; sorghum; ergot; disease.
Subjects:Technology > Technology (General)
Science > Statistics
Plant culture > Field crops > Grain. Cereals
Plant pests and diseases
Live Archive:12 Feb 2009 05:24
Last Modified:03 Sep 2021 16:43

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