Login | Request Account (DAF staff only)

Detection of Eimeria acervulina using the polymerase chain reaction

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Molloy, J.B., Eaves, F.W., Jeston, P.J., Minchin, C.M., Stewart, N.P., Lew, A.E. and Jorgensen, W.K. (1998) Detection of Eimeria acervulina using the polymerase chain reaction. Avian Diseases, 42 (1). pp. 119-123. ISSN 0005-2086

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link: https://doi.org/10.2307/1592583


A polymerase chain reaction (PCR)-based assay was developed for the detection of Eimeria acervulina. Primers were designed to amplify a fragment of the EASZ240/160 sporozoite antigen gene. The PCR assay detected as few as 10 E. acervulina oocysts in a mixed population containing a total of 106 oocysts. No nonspecific reaction was observed with any other species of avian Eimeria known to occur in Australia. PCR products from genomic DNA were 237 bp larger than predicted from previously reported cDNA sequences. Sequencing of the product revealed the presence of a probable intron. This work demonstrates the potential of PCR-based assays for identification and detection of avian Eimeria. Potential uses include identification of minor species present in mixed infections and quality control in the production of live vaccines.

Item Type:Article
Subjects:Veterinary medicine > Veterinary epidemiology. Epizootiology
Veterinary medicine > Diseases of special classes of animals > Avian
Live Archive:15 Mar 2024 03:54
Last Modified:15 Mar 2024 03:54

Repository Staff Only: item control page